pcDNAKv10.1mVenus
(Plasmid
#85705)
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PurposeKv10.1 fused mVenus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKCNH1
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Alt nameEag1
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SpeciesH. sapiens (human)
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Insert Size (bp)3600
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Entrez GeneKCNH1 (a.k.a. EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1)
- Promoter CMV
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Tag
/ Fusion Protein
- mVenus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAKv10.1mVenus was a gift from Luis Pardo (Addgene plasmid # 85705 ; http://n2t.net/addgene:85705 ; RRID:Addgene_85705) -
For your References section:
Functional K(v)10.1 channels localize to the inner nuclear membrane. Chen Y, Sanchez A, Rubio ME, Kohl T, Pardo LA, Stuhmer W. PLoS One. 2011 May 3;6(5):e19257. doi: 10.1371/journal.pone.0019257. 10.1371/journal.pone.0019257 PubMed 21559285
