pSGEMKv10.1
(Plasmid #85704)

• Purpose: Xenopus laevis oocyte expression of Kv10.1
• Depositing Lab: Luis Pardo
• Publication: Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

Backbone

• Vector backbone: pSGEM
• Vector type: Xenopus expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KCNH1
• Alt name: Eag1
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: KCNH1 (a.k.a. EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: pBluescript-KS

Resource Information

• Supplemental Documents:
  • pSGEMKv10.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSGEM vector, 3118 bp, engineered by Michael Hollmann & Dorothea Gremmels, March 1994. pSGEM is based on the pGEMHE-MCS vector (3096 bp) engineered by M.Hollmann. pGEMHE-MCS itself is based on the pGEMHE vector (3022 bp) which in turn is derived from pGEM-3Z of Promega (2743 bp) See also Liman,E.R., Tytgat,J. and Hess,P. Neuron (1992) Vol.9, pp.861-871