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Addgene

pcKv10.1
(Plasmid #85703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNH1
  • Alt name
    Eag1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    KCNH1 (a.k.a. EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcKv10.1 was a gift from Luis Pardo (Addgene plasmid # 85703 ; http://n2t.net/addgene:85703 ; RRID:Addgene_85703)

  • For your References section:

    Functional K(v)10.1 channels localize to the inner nuclear membrane. Chen Y, Sanchez A, Rubio ME, Kohl T, Pardo LA, Stuhmer W. PLoS One. 2011 May 3;6(5):e19257. doi: 10.1371/journal.pone.0019257. 10.1371/journal.pone.0019257 PubMed 21559285
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