HOXD9 (human) pET
(Plasmid
#8570)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXD9
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Alt nameHOX4C
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Alt nameHOX4.4
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SpeciesH. sapiens (human)
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Insert Size (bp)1059
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MutationPCR human cDNA clone; makes protein by in vitro TNT
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Entrez GeneHOXD9 (a.k.a. HOX4, HOX4C, Hox-4.3, Hox-5.2)
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Tag
/ Fusion Protein
- T7 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVincenzo Zappavigna and Fulvio Mavillio
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Zappavigna et al Genes and Development 8: 732-744; 1994
This is a synthetic version of HOXD9.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXD9 (human) pET was a gift from Corey Largman (Addgene plasmid # 8570 ; http://n2t.net/addgene:8570 ; RRID:Addgene_8570)
Map uploaded by the depositor.
