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Addgene

pAc-HT-Dmp53KRKR
(Plasmid #85699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1/V5 HIS A
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dmp53
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1200
  • Mutation
    K26R K302R
  • Entrez Gene
    p53 (a.k.a. Dmel_CG33336, CG10873, CG31325, CG33336, D-p53, DMP53, Dm-P53, DmP53, Dmel\CG33336, Dmp53, Dp53, P53, dmP53, dmp53, dp53, prac)
  • Promoter Ac5
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer AC5
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-HT-Dmp53KRKR was a gift from Licio Collavin & Giannino Del Sal (Addgene plasmid # 85699 ; http://n2t.net/addgene:85699 ; RRID:Addgene_85699)

  • For your References section:

    Modification of Drosophila p53 by SUMO modulates its transactivation and pro-apoptotic functions. Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky MH, Collavin L. J Biol Chem. 2008 Jul 25. 283(30):20848-56. 10.1074/jbc.M710186200 PubMed 18492669
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