HOXD8 (human,partial) HIS-tag pET
(Plasmid #8569)

• Depositing Lab: Corey Largman
• Publication: HOX protein coding clones not referenced in publications (unpublished)

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXD8
• Alt name: HOX4E
• Alt name: HOX4.3
• Alt name: HOX5.4
• Species: H. sapiens (human)
• Insert Size (bp): 298
• Mutation: Partial protein containing 4 AA N-terminal to HD and 11 AA C-terminal to HD; makes protein by Western
• GenBank ID: X15507
• Entrez Gene: HOXD8 (a.k.a. HOX4, HOX4E, HOX5.4)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: partial genomic clone from Edoardo Boncinelli

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry