pmCherry-Gal3
(Plasmid #85662)

• Purpose: Fluorescent reporter for lysosomal damage
• Depositing Lab: Hemmo Meyer
• Publication: Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Backbone

• Vector backbone: pmCherry-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LGALS3
• Alt name: Galectin-3
• Alt name: Gal3
• Species: H. sapiens (human)
• GenBank ID: NM_002306.3 NM_001177388.1
• Entrez Gene: LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR amplification of the Gal3 coding sequence from U2OS cDNA