pMSCV-SMYD3
(Plasmid
#85650)
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Purposeretroviral expression of human SMYD3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMYD3
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Alt nameSET and MYND domain containing 3
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SpeciesH. sapiens (human)
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Entrez GeneSMYD3 (a.k.a. KMT3E, ZMYND1, ZNFN3A1, bA74P14.1)
- Promoter LTR
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Tag
/ Fusion Protein
- Flag
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-SMYD3 was a gift from Julien Sage (Addgene plasmid # 85650 ; http://n2t.net/addgene:85650 ; RRID:Addgene_85650) -
For your References section:
SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer. Mazur PK, Reynoird N, Khatri P, Jansen PW, Wilkinson AW, Liu S, Barbash O, Van Aller GS, Huddleston M, Dhanak D, Tummino PJ, Kruger RG, Garcia BA, Butte AJ, Vermeulen M, Sage J, Gozani O. Nature. 2014 Jun 12;510(7504):283-7. doi: 10.1038/nature13320. Epub 2014 May 21. 10.1038/nature13320 PubMed 24847881