HOXC6 (human) HIS-tag pET
(Plasmid #8565)

• Depositing Lab: Corey Largman
• Publication: HOX protein coding clones not referenced in publications (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXC6
• Alt name: HOX3C
• Alt name: HOX3.3
• Alt name: HHO.c8
• Species: H. sapiens (human)
• Insert Size (bp): 460
• Mutation: This protein starts at an internal Met initially assigned by Simeone et al and encodes a 152 AA protein missing the initial 82 AA reported in the annotation; makes protein by Western
• GenBank ID: NM_004503
• Entrez Gene: HOXC6 (a.k.a. CP25, HHO.C8, HOX3, HOX3C)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fulvio Mavillio

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Simeone et al. Proc. Natl. Acad. USA 84: 4914-4918, 1987