HOXC5 (human) HIS-tag pET
(Plasmid
#8563)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXC5
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Alt nameHOX3D
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Alt nameHOX3D
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Alt nameHOX3.4
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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MutationStarts at Amino Acid #2 (Ser); makes protein by Western
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GenBank IDX61755
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Entrez GeneHOXC5 (a.k.a. CP11, HOX3, HOX3D)
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BglII/XhoI blunt (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFulvio Mavillio
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Arcioni et al EMBO J. 11: 265-277, 1992
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXC5 (human) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8563 ; http://n2t.net/addgene:8563 ; RRID:Addgene_8563)
Map uploaded by the depositor.
