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Addgene

Munc18cwt-pDEST27
(Plasmid #85626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST27
  • Backbone size w/o insert (bp) 8100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Munc18c
  • Alt name
    syntaxin binding protein 3, stxbp3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1717
  • Entrez Gene
    Stxbp3 (a.k.a. Munc-18c, Stxbp3a, Sxtbp3, Unc18-3)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pDEST from Invitrogen. Munc18c was originally cloned by the depositing scientist.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Munc18cwt-pDEST27 was a gift from David James (Addgene plasmid # 85626 ; http://n2t.net/addgene:85626 ; RRID:Addgene_85626)

  • For your References section:

    Molecular dissection of the Munc18c/syntaxin4 interaction: implications for regulation of membrane trafficking. Latham CF, Lopez JA, Hu SH, Gee CL, Westbury E, Blair DH, Armishaw CJ, Alewood PF, Bryant NJ, James DE, Martin JL. Traffic. 2006 Oct;7(10):1408-19. Epub 2006 Aug 10. 10.1111/j.1600-0854.2006.00474.x PubMed 16899085
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