Munc18cF119E-pDEST27
(Plasmid #85625)

• Purpose: GST tagged Munc18c with a F119E mutation
• Depositing Lab: David James
• Publication: Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 2006 Aug 10.

Backbone

• Vector backbone: pDEST27
• Backbone size w/o insert (bp): 8100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Munc18c
• Alt name: syntaxin binding protein 3, stxbp3
• Species: M. musculus (mouse)
• Insert Size (bp): 1717
• Mutation: F119E
• Entrez Gene: Stxbp3 (a.k.a. Munc-18c, Stxbp3a, Sxtbp3, Unc18-3)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDEST from Invitrogen. Munc18c was originally cloned by the depositing scientist.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry