5XDRE-luciferase
(Plasmid
#85623)
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PurposeLuciferase reporter containing five tandem copies of the core Dpp response element
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-Basic Vector
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 6318
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name5x DRE
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Insert Size (bp)1500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer N\A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5XDRE-luciferase was a gift from Kenneth Irvine (Addgene plasmid # 85623 ; http://n2t.net/addgene:85623 ; RRID:Addgene_85623) -
For your References section:
Cooperative regulation of growth by Yorkie and Mad through bantam. Oh H, Irvine KD. Dev Cell. 2011 Jan 18;20(1):109-22. doi: 10.1016/j.devcel.2010.12.002. 10.1016/j.devcel.2010.12.002 PubMed 21238929
