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Addgene

pUAST-yki:FLAG(3x)
(Plasmid #85622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Total vector size (bp) 10304
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yki
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1400
  • Entrez Gene
    yki (a.k.a. Dmel_CG4005, CG4005, Dmel\CG4005, YAP, YKI, Yki, yap)
  • Tag / Fusion Protein
    • 3x FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer N\A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-yki:FLAG(3x) was a gift from Kenneth Irvine (Addgene plasmid # 85622 ; http://n2t.net/addgene:85622 ; RRID:Addgene_85622)

  • For your References section:

    In vivo regulation of Yorkie phosphorylation and localization. Oh H, Irvine KD. Development. 2008 Mar;135(6):1081-8. doi: 10.1242/dev.015255. Epub 2008 Feb 6. 10.1242/dev.015255 PubMed 18256197
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