pUAST-attB-GFP-V5-His
(Plasmid
#85621)
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PurposeExpresses GFP and V5 in Drosophila.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUAST-attb
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
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Insert Size (bp)717
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Tag
/ Fusion Protein
- V5, His
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N\A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST-attB-GFP-V5-His was a gift from Kenneth Irvine (Addgene plasmid # 85621 ; http://n2t.net/addgene:85621 ; RRID:Addgene_85621) -
For your References section:
Zyxin links fat signaling to the hippo pathway. Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD. PLoS Biol. 2011 Jun;9(6):e1000624. doi: 10.1371/journal.pbio.1000624. Epub 2011 Jun 7. 10.1371/journal.pbio.1000624 PubMed 21666802
