Skip to main content
Addgene

HOXA5 (human) HIS-tag pET
(Plasmid #8562)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA5
  • Alt name
    HOX1C
  • Alt name
    HOX1.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    811
  • Mutation
    SacI site at amino acids 1-2 was used to clone into pET; makes protein by western
  • Entrez Gene
    HOXA5 (a.k.a. HOX1, HOX1.3, HOX1C)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Judith Gasson

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA5 (human) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8562 ; http://n2t.net/addgene:8562 ; RRID:Addgene_8562)
Related items:
Commonly requested with: