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pUAST-attB-yki:V5-S250A
(Plasmid #85618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-attb
  • Modifications to backbone
    PCR fragment of Yki was subcloned into pAC5.1/V5 HisA, then KpnI and PmeI fragment was subcloned into pUAST using KpnI and XbaI.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yki-V5-S250A
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1500
  • Tag / Fusion Protein
    • V5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer N\A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-attB-yki:V5-S250A was a gift from Kenneth Irvine (Addgene plasmid # 85618 ; http://n2t.net/addgene:85618 ; RRID:Addgene_85618)

  • For your References section:

    In vivo analysis of Yorkie phosphorylation sites. Oh H, Irvine KD. Oncogene. 2009 Apr 30;28(17):1916-27. doi: 10.1038/onc.2009.43. Epub 2009 Mar 30. 10.1038/onc.2009.43 PubMed 19330023
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