pAW019-2
(Plasmid #85614)

• Purpose: Inducible dCas9 integration vector for Bacillus subtilis
• Depositing Lab: Perry Chou
• Publication: Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16.

Backbone

• Vector backbone: pAX01 (ColE1)
• Backbone size w/o insert (bp): 7800
• Total vector size (bp): 12000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): HI-Control 10G
• Growth instructions: Use 0.5 ug/mL erythromycin for cloning in B. subtilis
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dcas9
• Species: S. pyogenes
• Insert Size (bp): 4107
• Promoter: PxylA (B. megaterium)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SbfI (not destroyed)
• 5′ sequencing primer: AAGATAGTTGATGGATAAACTTGTTCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pdCas9-bacteria, Stanley Qi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry