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Addgene

HOXD1 (murine, partial) HIS-tag pET
(Plasmid #8560)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8560 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXD1
  • Alt name
    HOX4G
  • Alt name
    HOX4.9
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    360
  • Mutation
    Partial protein containing 10 AA N-terminal to HD and 40 AA C-terminal to HD; makes protein by Western
  • GenBank ID
    X60034
  • Entrez Gene
    Hoxd1 (a.k.a. Hox-4.9)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RT-PCR from murine embryonic tissue

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXD1 (murine, partial) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8560 ; http://n2t.net/addgene:8560 ; RRID:Addgene_8560)
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