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Purpose(Empty Backbone) An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 85586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMA-T
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Backbone manufacturerLife Technologies
- Backbone size (bp) 2380
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Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Forward, T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gRNA Cloning Vector Bbs I ver. 2 was a gift from Hodaka Fujii (Addgene plasmid # 85586 ; http://n2t.net/addgene:85586 ; RRID:Addgene_85586) -
For your References section:
Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus. Fujita T, Yuno M, Fujii H. Sci Rep. 2016 Jul 28;6:30485. doi: 10.1038/srep30485. 10.1038/srep30485 PubMed 27465215
Map uploaded by the depositor.
