HOXA6 (murine, partial) HIS-tag pET
(Plasmid
#8558)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 8558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET28a
- Backbone size w/o insert (bp) 5400
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHOXA6
-
Alt nameHOX1B
-
Alt nameHOX1.2
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)295
-
Mutationpartial cDNA encoding homeodomain and preceeding 18 N-terminal AA and final 18 C-terminal AA; makes protein by Western
-
Entrez GeneHoxa6 (a.k.a. Hox-1.2)
-
Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HOXA6 (murine, partial) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8558 ; http://n2t.net/addgene:8558 ; RRID:Addgene_8558)
Map uploaded by the depositor.