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Addgene

HOXA1 (murine) HIS-tag pET
(Plasmid #8556)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA1
  • Alt name
    HOX1F
  • Alt name
    HOX1.6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    400
  • Mutation
    clone contains 130 AA including the homeodomain, but is missing ~205 AA from the longest known protein encoded by HOXA1, makes protein by western
  • GenBank ID
    M22115
  • Entrez Gene
    Hoxa1 (a.k.a. ERA1, Hox-1.6)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA1 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8556 ; http://n2t.net/addgene:8556 ; RRID:Addgene_8556)
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