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HOXD13 (chicken) HIS-tag pET
(Plasmid #8555)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXD13
  • Alt name
    HOX4I
  • Alt name
    HOX4.8
  • Insert Size (bp)
    1970
  • Mutation
    contains 10 nt 5' to ATG start; makes protein by Western
  • GenBank ID
    L09550
  • Entrez Gene
    HOXD13
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    William Holt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rogina et al Nuc. Acids Res. 21: 1316 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXD13 (chicken) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8555 ; http://n2t.net/addgene:8555 ; RRID:Addgene_8555)

  • For your References section:

    The Abd-B-like Hox homeodomain proteins can be subdivided by the ability to form complexes with Pbx1a on a novel DNA target. Shen WF, Rozenfeld S, Lawrence HJ, Largman C. J Biol Chem 1997 Mar 28;272(13):8198-206. 10.1074/jbc.272.13.8129 PubMed 9079637
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