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Addgene

HOXD12 (murine) pRSETa
(Plasmid #8554)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETa
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXD12
  • Alt name
    HOX4H
  • Alt name
    HOX4.7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    838
  • Mutation
    Fusion sequence is: ggatccATG
  • GenBank ID
    MMHOX47
  • Entrez Gene
    Hoxd12 (a.k.a. Hox-4.7, Hox-5.6)
  • Tags / Fusion Proteins
    • XPress (N terminal on backbone)
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fusion tags can be removed using enterokinase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXD12 (murine) pRSETa was a gift from Corey Largman (Addgene plasmid # 8554 ; http://n2t.net/addgene:8554 ; RRID:Addgene_8554)

  • For your References section:

    AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
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