HOXD12 (murine) HIS-tag pET
(Plasmid
#8553)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXD12
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Alt nameHOX4H
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Alt nameHOX4.7
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SpeciesM. musculus (mouse)
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Insert Size (bp)838
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MutationFusion sequence is: ggatccATG
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GenBank IDMMHOX47
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Entrez GeneHoxd12 (a.k.a. Hox-4.7, Hox-5.6)
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXD12 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8553 ; http://n2t.net/addgene:8553 ; RRID:Addgene_8553) -
For your References section:
AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
Map uploaded by the depositor.
