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Addgene

pET28a-sfGFP
(Plasmid #85492)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85492 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6150
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    superfolder GFP
  • Alt name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492 ; http://n2t.net/addgene:85492 ; RRID:Addgene_85492)

  • For your References section:

    Site-specific incorporation of unnatural amino acids as probes for protein conformational changes. Peeler JC, Mehl RA. Methods Mol Biol. 2012;794:125-34. doi: 10.1007/978-1-61779-331-8_8. 10.1007/978-1-61779-331-8_8 PubMed 21956560
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