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Addgene

pCDNA3 IRF7(5’UTR)-FF
(Plasmid #85490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7362
  • Modifications to backbone
    murine IRF7 5’ UTR was inserted into pCDNA3-Firefly Luciferase plasmid using BamHI.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'UTR of IRF7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    362
  • GenBank ID
    NM_016850.3
  • Entrez Gene
    Irf7
  • Tag / Fusion Protein
    • Luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IRF7 5'UTR was inserted before luciferase ATG in pCDNA3 Firefly luciferase from Addgene (#26612)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 IRF7(5’UTR)-FF was a gift from Ivan Topisirovic (Addgene plasmid # 85490 ; http://n2t.net/addgene:85490 ; RRID:Addgene_85490)

  • For your References section:

    nanoCAGE reveals 5' UTR features that define specific modes of translation of functionally related MTOR-sensitive mRNAs. Gandin V, Masvidal L, Hulea L, Gravel SP, Cargnello M, McLaughlan S, Cai Y, Balanathan P, Morita M, Rajakumar A, Furic L, Pollak M, Porco JA Jr, St-Pierre J, Pelletier J, Larsson O, Topisirovic I. Genome Res. 2016 May;26(5):636-48. doi: 10.1101/gr.197566.115. Epub 2016 Mar 16. 10.1101/gr.197566.115 PubMed 26984228
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