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Addgene

HOXD4 (murine) MSCV
(Plasmid #8549)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXD4
  • Alt name
    HOX4B
  • Alt name
    HOX4.2
  • Alt name
    HHO.c13
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    905
  • Mutation
    contains T7 and HIS tags from pET; makes protein by Western
  • GenBank ID
    J03770
  • Entrez Gene
    Hoxd4 (a.k.a. 6030436D05Rik, Hox-4.2, Hox-5.1)
  • Tags / Fusion Proteins
    • T7 (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI/HpaI blunt (destroyed during cloning)
  • 3′ cloning site XbaI/HpaI blunt (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mark Featherstone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Popperl and Featherstone, EMBO J:11, 3673-80, 1992

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXD4 (murine) MSCV was a gift from Corey Largman (Addgene plasmid # 8549 ; http://n2t.net/addgene:8549 ; RRID:Addgene_8549)

  • For your References section:

    Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C. Nucleic Acids Res 1996 Mar 1;24(5):898-906. 10.1093/nar/24.5.898 PubMed 8600458
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