pCDNA3 ATP5O(TISU)-FF
(Plasmid #85489)

• Purpose: Firefly luciferase under the control of ATP5O TISU
• Depositing Lab: Ivan Topisirovic
• Publication: Gandin et al Genome Res. 2016 May;26(5):636-48. doi: 10.1101/gr.197566.115. Epub 2016 Mar 16.

Backbone

• Vector backbone: pCDNA3 addgene #26612
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 7008
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Full TISU element of ATP5O
• Species: H. sapiens (human)
• Insert Size (bp): 8
• Mutation: 2nd and 3rd codon of luciferase were modified from GAAGAC to GCTGCC
• GenBank ID: NM_001697.2
• Entrez Gene: ATP5PO (a.k.a. ATP5O, ATPO, HMC08D05, MC5DN7, OSCP)
• Tag / Fusion Protein:
  • Firefly luciferase (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry