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Addgene

pCDNA3 NDUFS6(5’UTR)-FF
(Plasmid #85488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3 addgene #26612
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7022
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'UTR of NDUFS6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    23
  • GenBank ID
    NM_004553.4
  • Entrez Gene
    NDUFS6 (a.k.a. CI-13kA, CI13KDA, CI-13kD-A)
  • Tag / Fusion Protein
    • Firefly luciferase (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 NDUFS6(5’UTR)-FF was a gift from Ivan Topisirovic (Addgene plasmid # 85488 ; http://n2t.net/addgene:85488 ; RRID:Addgene_85488)

  • For your References section:

    nanoCAGE reveals 5' UTR features that define specific modes of translation of functionally related MTOR-sensitive mRNAs. Gandin V, Masvidal L, Hulea L, Gravel SP, Cargnello M, McLaughlan S, Cai Y, Balanathan P, Morita M, Rajakumar A, Furic L, Pollak M, Porco JA Jr, St-Pierre J, Pelletier J, Larsson O, Topisirovic I. Genome Res. 2016 May;26(5):636-48. doi: 10.1101/gr.197566.115. Epub 2016 Mar 16. 10.1101/gr.197566.115 PubMed 26984228
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