pRSETB-de4GFP
(Plasmid #85472)

• Purpose: Inducible expression of dual emitting pH sensitive GFP in bacterial cells
• Depositing Lab: S. James Remington
• Publication: Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-88.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 3600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: de4GFP
• Species: jellyfish
• Insert Size (bp): 714
• Mutation: S65T/C48S/H148C/T203C
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Tsien

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry