mCer-TG2-YFP
(Plasmid #85471)

• Purpose: TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET
• Depositing Labs: Jeffrey Keillor, Ray Truant
• Publication: Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371/journal.pone.0044159. Epub 2012 Aug 31.

Backbone

• Vector backbone: mCerulean-C1
• Modifications to backbone: eYFP insert cloned into BamHI and XbaI sites at the 3' end of the multiple cloning site (MCS).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hTG2
• Alt name: transglutaminase 2
• Species: H. sapiens (human)
• Entrez Gene: TGM2 (a.k.a. G(h), TG(C), TGC, hTG2, tTG)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCerulean (N terminal on backbone)
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: Acc65I (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The hTG2 insert contains V224G and A141T mutations. The depositor states that these mutations do not affect plasmid function.