p-mCherry-C1-PsACR1
(Plasmid #85465)

• Purpose: Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)
• Depositing Lab: Peter Hegemann
• Publication: Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7. doi: 10.1074/jbc.M115.699637. Epub 2016 Jan 6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4703
• Total vector size (bp): 5576
• Modifications to backbone: EGFP has been replaced by mCherry using AgeI/XhoI (XhoI site destroyed during cloning)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synthetic construct PsChR1 gene
• Alt name: PsACR1
• Species: Synthetic; Proteomonas sulcata
• Insert Size (bp): 873
• GenBank ID: KF992074.1
• Promoter: CMV (+enhancer)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: AgeI (unknown if destroyed)
• 5′ sequencing primer: EGFP-N1-F: GAGGTCTATATAAGCAGAGC or CMV-F: GCAAATGGGCGGTAGGCGT
• 3′ sequencing primer: EGFP-C1-R: AACCATTATAAGCTGCAATAAAC

Resource Information

• Supplemental Documents:
  • 265_PsACR1-mCherry.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry