pLPT20
(Plasmid #85460)

• Purpose: Repressilator with integrated reporter
• Depositing Lab: Johan Paulsson
• Publication: Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.

Backbone

• Vector backbone: pSC101
• Total vector size (bp): 8766
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mCfp
• Promoter: PRNAI

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGACGGCCAGTGAATTCGAGC

Gene/Insert 2

• Gene/Insert name: mVenus
• Promoter: PLtet

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcttagacgtactcgagcacgagg

Gene/Insert 3

• Gene/Insert name: tetR
• Promoter: PLlac
• Tag / Fusion Protein:
  • ssrA (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: cctcgtgctcgagtacgtctaag

Gene/Insert 4

• Gene/Insert name: lacI
• Promoter: PR
• Tag / Fusion Protein:
  • ssrA (C terminal on insert)

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: gaaaactacgctttagcagcttaatctag

Gene/Insert 5

• Gene/Insert name: cI
• Promoter: PLtet
• Tag / Fusion Protein:
  • ssrA (C terminal on insert)

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: gcaacaccagaacagcccg

Resource Information

• Supplemental Documents:
  • pLPT20.gbk
• A portion of this plasmid was derived from a plasmid made by: Repressilator from Michael Elowitz

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry