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HOXC4 (human) MSCV
(Plasmid #8546)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXC4
  • Alt name
    HOX3E
  • Alt name
    cp19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    914
  • Mutation
    Contains improved ribosomal binding site and NcoI site at ATG start; I2V mutation, truncated at aa247 when compared to CAA30376.1
  • GenBank ID
    X07495 CAA30376.1
  • Entrez Gene
    HOXC4 (a.k.a. HOX3, HOX3E, cp19)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5’
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Edoardo Boncinelli

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Simeone et al Nucl. Acids Res. 16: 5379-90, 1988

Please note that the HOXC4 insert in this plasmid is truncated at amino acid 247 when compared to GenBank reference sequence CAA30376.1 and Figure 3 of the NAR publication listed above.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXC4 (human) MSCV was a gift from Corey Largman (Addgene plasmid # 8546 ; http://n2t.net/addgene:8546 ; RRID:Addgene_8546)

  • For your References section:

    Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C. Nucleic Acids Res 1996 Mar 1;24(5):898-906. 10.1093/nar/24.5.898 PubMed 8600458
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