Diacylglycerol Kinase Zeta
(Plasmid
#85454)
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Purposeexpression of Diacylglycerol Kinase Zeta
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 (+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDiacylglycerol Kinase Zeta
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SpeciesH. sapiens (human)
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Entrez GeneDGKZ (a.k.a. DAGK5, DAGK6, DGK-ZETA, hDGKzeta)
- Promoter CMV
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Tag
/ Fusion Protein
- 3x HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Diacylglycerol Kinase Zeta was a gift from Stephen Gee (Addgene plasmid # 85454 ; http://n2t.net/addgene:85454 ; RRID:Addgene_85454) -
For your References section:
Interaction of gamma 1-syntrophin with diacylglycerol kinase-zeta. Regulation of nuclear localization by PDZ interactions. Hogan A, Shepherd L, Chabot J, Quenneville S, Prescott SM, Topham MK, Gee SH. J Biol Chem. 2001 Jul 13;276(28):26526-33. Epub 2001 May 14. 10.1074/jbc.M104156200 PubMed 11352924
