pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
(Plasmid #85453)

• Purpose: Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.
• Depositing Lab: Aviv Regev
• Publication: Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052.

Backbone

• Vector backbone: pLKO
• Total vector size (bp): 13840
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6-sgRNA-Backbone-UP-TdTomato
• Species: Synthetic
• Insert Size (bp): 6913
• Mutation: deletion of AA123-365 in tdTomato (please see depositor comment below)
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that deletion of AA123-365 in tdTomato was found during Addgene's quality control. The depositor noted that this deletion does NOT affect plasmid function.

Any issue or question regarding this vector can be also directed to Le Cong (Regev lab) at .