pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
(Plasmid #85452)

• Purpose: Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
• Depositing Lab: Aviv Regev
• Publication: Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052.

Backbone

• Vector backbone: pLKO
• Backbone size w/o insert (bp): 6927
• Total vector size (bp): 12075
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
• Alt name: SauCas9, sgRNA scaffold
• Species: Synthetic; S aureus
• Insert Size (bp): 5148
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Any issue or question regarding this vector can be also directed to Le Cong (Regev lab) at .