HOXB13 (human) MSCV
(Plasmid
#8544)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 8544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneMSCV
- Backbone size w/o insert (bp) 6400
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHOXB13
-
Alt nameSDX1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1160
-
Mutationcontains 141 NT of 5' UTR prior to ATG start
-
Entrez GeneHOXB13 (a.k.a. HPC9, PSGD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI/SmaI blunt (destroyed during cloning)
- 3′ cloning site BamHI/BglII blunt (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HOXB13 (human) MSCV was a gift from Corey Largman (Addgene plasmid # 8544 ; http://n2t.net/addgene:8544 ; RRID:Addgene_8544) -
For your References section:
Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless fetal wounds. Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C. J Invest Dermatol 1998 Jul;111(1):57-63. 10.1046/j.1523-1747.1998.00238.x PubMed 9665387
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/27/08/0a7154a6-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)