HOXB13 (human) HIS-tag pET
(Plasmid #8543)

• Depositing Lab: Corey Largman
• Publication: Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HOXB13
• Alt name: SDX1
• Species: H. sapiens (human)
• Insert Size (bp): 1019
• Mutation: makes protein by Western and by transcription/translation
• Entrez Gene: HOXB13 (a.k.a. HPC9, PSGD)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI/EcoRI blunt (destroyed during cloning)
• 3′ cloning site: EcoRI in TA Vector to HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry