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Addgene

HOXB9 (murine) pSG5
(Plasmid #8542)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8542 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HOXB9
  • Alt name
    HOX2E
  • Alt name
    HOX2.5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    Contains cDNA encoding 11 amio acids from the 5'untranslated region preceeding the ATG start
  • GenBank ID
    M34857
  • Entrez Gene
    Hoxb9 (a.k.a. Hox-2.5)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI/BglII blunt (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    William McGinnis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB9 (murine) pSG5 was a gift from Corey Largman (Addgene plasmid # 8542)

  • For your References section:

    Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus. Chang CP, Brocchieri L, Shen WF, Largman C, Cleary ML. Mol Cell Biol 1996 Apr;16(4):1734-45. PubMed 8657149
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