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Addgene

pGH224_sgRNA_2xMS2_Puro
(Plasmid #85413)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85413 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #61427
  • Modifications to backbone
    replaced Zeocin resistance with puromycin resistance
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    puromycin resistance
  • gRNA/shRNA sequence
    template

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original addgene plasmid #61427 from Feng Zhang lab was modified by replacing Zeocin resistance with puromycin resistance
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGH224_sgRNA_2xMS2_Puro was a gift from Michael Bassik (Addgene plasmid # 85413 ; http://n2t.net/addgene:85413 ; RRID:Addgene_85413)

  • For your References section:

    Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. 10.1038/nmeth.4038 PubMed 27798611
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