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Addgene

pGH314_wtGFP S65T, Q80H
(Plasmid #85407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85407 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSicoR_no puro
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    wtGFP S65T, Q80H

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGH314_wtGFP S65T, Q80H was a gift from Michael Bassik (Addgene plasmid # 85407 ; http://n2t.net/addgene:85407 ; RRID:Addgene_85407)

  • For your References section:

    Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. 10.1038/nmeth.4038 PubMed 27798611
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