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Addgene

RGD-I27-CC
(Plasmid #85393)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85393 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) strain for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
  • Alt name
    KS29-ECC
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fibronectin binding motif (RGD) is TVYAVTGRGDSPASSAA.

A TAG (amber) codon was added between the RGD motif and I27 for labeling with p-azidophenylalanine, an unnatural amino acid. The RGD-I27-CC fusion protein can be labeled with the Cy3 dye at this site using Cu-free click cyanoaddition coupling.

I27 domain is the 27th immunoglobulin domain of cardiac titin. Two cysteine residues were added at the C-terminus of I27 to allow immobilization onto a gold nanoparticle.

Please see the associated publication for material and methods information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RGD-I27-CC was a gift from Khalid Salaita (Addgene plasmid # 85393 ; http://n2t.net/addgene:85393 ; RRID:Addgene_85393)
  • For your References section:

    Titin-Based Nanoparticle Tension Sensors Map High-Magnitude Integrin Forces within Focal Adhesions. Galior K, Liu Y, Yehl K, Vivek S, Salaita K. Nano Lett. 2016 Jan 13;16(1):341-8. doi: 10.1021/acs.nanolett.5b03888. Epub 2015 Dec 7. 10.1021/acs.nanolett.5b03888 PubMed 26598972