HIV-1 Gag-mCherry
(Plasmid #85390)

• Purpose: Visualization marker for viral core to measure cellular uptake
• Depositing Lab: Gregory Melikyan
• Publication: Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. doi: 10.1042/BJ20101588.

Backbone

• Vector backbone: pcDNA3.1/Zeo (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 gag
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 2214
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pcDNA3.1zeo+GagCherry.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gag fragment was amplified from p96ZM651gag-opt vector [NIH AIDS Research and Reference Reagent Program] and cloned into pcDNA3.1/Zeo(+) using HindIII and BamHI sites. mCherry fragment was amplified from pRSET-BmCherry (from Dr R. Tsien, University of California, San Diego) and cloned into pcDNA3.1/Zeo(+) using BamHI and XhoI sites.