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HOXB7 (human) pCMV
(Plasmid #8539)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV1
  • Backbone size w/o insert (bp) 4820
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB7
  • Alt name
    HOX2C
  • Alt name
    HOX2.3
  • Alt name
    HHO.c1.95
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    753
  • Mutation
    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile-Leu-Ala linker; makes protein Western
  • Entrez Gene
    HOXB7 (a.k.a. HHO.C1, HOX2, HOX2C, Hox-2.3)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI/HIndIII blunt (destroyed during cloning)
  • 3′ cloning site HindIII/HindII blunt (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Edoardo Boncinelli

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Simeone et al. Proc. Natl. Acad. Sci. USA 84: 4914-18, 1987

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB7 (human) pCMV was a gift from Corey Largman (Addgene plasmid # 8539 ; http://n2t.net/addgene:8539 ; RRID:Addgene_8539)

  • For your References section:

    Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
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