HOXB7 (human) pCMV
(Plasmid
#8539)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV1
- Backbone size w/o insert (bp) 4820
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB7
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Alt nameHOX2C
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Alt nameHOX2.3
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Alt nameHHO.c1.95
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SpeciesH. sapiens (human)
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Insert Size (bp)753
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Mutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile-Leu-Ala linker; makes protein Western
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Entrez GeneHOXB7 (a.k.a. HHO.C1, HOX2, HOX2C, Hox-2.3)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI/HIndIII blunt (destroyed during cloning)
- 3′ cloning site HindIII/HindII blunt (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEdoardo Boncinelli
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Simeone et al. Proc. Natl. Acad. Sci. USA 84: 4914-18, 1987
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB7 (human) pCMV was a gift from Corey Largman (Addgene plasmid # 8539 ; http://n2t.net/addgene:8539 ; RRID:Addgene_8539) -
For your References section:
Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/04/77/08dd1b5c-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)