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Addgene

HOXB7 (human) FLAG pSP65
(Plasmid #8537)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3005
  • Vector type
    Bacterial Expression ; In vitro transcription/translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB7
  • Alt name
    HOX2C
  • Alt name
    HOX2.3
  • Alt name
    HHO.c1.95
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    828
  • Mutation
    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile-Leu-Ala linker; makes protein by in vitro transcription/translation
  • Entrez Gene
    HOXB7 (a.k.a. HHO.C1, HOX2, HOX2C, Hox-2.3)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Edoardo Boncinelli
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

can be used for in vitro TNT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB7 (human) FLAG pSP65 was a gift from Corey Largman (Addgene plasmid # 8537 ; http://n2t.net/addgene:8537 ; RRID:Addgene_8537)

  • For your References section:

    Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
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