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Addgene

HOXB6 (human) FLAG pMSCV
(Plasmid #8534)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP for FACS sorting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB6
  • Alt name
    HOX2B
  • Alt name
    HOX2.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    771
  • Mutation
    contains improved ribosomal binding site and FLAG epitope tag on N-terminal, starts at AA; #2, makes protein by western; contains GFP driven from an IRES
  • GenBank ID
    X58431 BC014651
  • Entrez Gene
    HOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI/HpaI blunt (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB6 (human) FLAG pMSCV was a gift from Corey Largman (Addgene plasmid # 8534 ; http://n2t.net/addgene:8534 ; RRID:Addgene_8534)

  • For your References section:

    Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
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