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Addgene

HOXB4 (human) HIS-tag pET new RBS
(Plasmid #8525)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB4
  • Alt name
    HOX2F
  • Alt name
    HOX2.6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    959
  • Mutation
    Contains improved Ribosomal binding site; makes protein by Western
  • GenBank ID
    NM_024015
  • Entrez Gene
    HOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Edoardo Boncinelli

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB4 (human) HIS-tag pET new RBS was a gift from Corey Largman (Addgene plasmid # 8525 ; http://n2t.net/addgene:8525 ; RRID:Addgene_8525)

  • For your References section:

    Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
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