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HOXB3 (human) FLAG pSP65
(Plasmid #8523)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3005
  • Vector type
    Bacterial Expression ; In vitro transcription/translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB3
  • Alt name
    HOX2G
  • Alt name
    HOX2.7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1644
  • Mutation
    Contains 5 additional amino acids before ATG from cloning vectors; makes protein by Western
  • GenBank ID
    X16667
  • Entrez Gene
    HOXB3 (a.k.a. HOX2, HOX2G, Hox-2.7)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI in Bsk linker (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Guy Sauvageau

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

used to make protein in vitro

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB3 (human) FLAG pSP65 was a gift from Corey Largman (Addgene plasmid # 8523 ; http://n2t.net/addgene:8523 ; RRID:Addgene_8523)

  • For your References section:

    Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C. Nucleic Acids Res 1996 Mar 1;24(5):898-906. 10.1093/nar/24.5.898 PubMed 8600458
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